Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

A central core structure in an antibody variable domain determines antigen specificity

In English

Författare

  • P. Jirholt
  • L. Strandberg
  • B. Jansson
  • E. Krambovitis
  • Eskil Söderlind
  • Carl Borrebaeck
  • Roland Carlsson
  • L. Danielsson
  • Mats Ohlin

Summary, in English

Antibody binding sites provide an adaptable surface capable of interacting with essentially any molecular target. Using CDR shuffling, residues important for the assembly of mucin-1 specific paratopes were defined by random recombination of the complementarity determining regions derived from a set of mucin-1 specific clones, previously selected from an antibody fragment library. It was found that positions 33 and 50 in the heavy chain and 32, 34, 90, 91 and 96 in the light chain were conserved in many of the clones. These particular residues seem to be located centrally in the binding site as indicated by a structure model analysis. The importance of several of these conserved residues was supported by their presence in a mouse monoclonal antibody with a known structure and the same epitope specificity. Several of these corresponding residues in the mouse monoclonal antibody are known to interact with the antigen. In conclusion, critical residues important for maintaining a human antigen-specific binding site during the process of in vitro antibody evolution were defined. Furthermore, an explanation for the observed restricted germline gene usage in certain antibody responses against protein epitopes is provided.

Publiceringsår

2001

Språk

Engelska

Sidor

67-74

Publikation/Tidskrift/Serie

Protein Engineering

Volym

14

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Oxford University Press

Ämne

  • Immunology in the medical area

Nyckelord

  • antibody evolution
  • complementarity determining region
  • mucin-1
  • paratope
  • phage display
  • DETERMINING REGION CDR
  • CHAIN FV ANTIBODIES
  • MONOCLONAL-ANTIBODIES
  • SOMATIC HYPERMUTATION
  • IMMUNE-RESPONSE
  • BINDING-SITE
  • IN-VITRO
  • GENES
  • REPERTOIRE
  • DIVERSITY

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1460-213X