Epidemiological MIC cut-off values for tigecycline calculated from Etest MIC values using normalized resistance interpretation
Författare
Summary, in English
Objectives: To apply the normalized resistance interpretation (NRI) method to Etest MIC results which have higher precision than conventional log(2) dilution MIC tests due to the inclusion of intermediate values. If successful, NRI might provide an objective tool for the definition of epidemiological MIC cut-off values. Methods: MICs of tigecycline and other antimicrobial agents were determined for 4771 clinical isolates comprising five Gram-positive and 13 Gram-negative species or species groups using the Etest. Histograms of MIC values were constructed for each species and NRI calculations were applied to them. An upper MIC limit of 2.5 SD above the theoretical mean of the normalized distribution was used for setting the epidemiological cut-off values. Results: Calculated cut-off values for wild-type strains were between 0.11 and 0.96 mg/L for Gram-positive species, and between 0.44 and 8.3 mg/L for Gram-negative species, except for Pseudomonas aeruginosa, which had a cut-off value of 450 mg/L, consistent with earlier reports on the lack of activity of tigecycline against this species. Conclusions: NRI offers an objective method for the analysis of MICs produced using Etests and the determination of epidemiological MIC cut-off values.
Avdelning/ar
Publiceringsår
2006
Språk
Engelska
Sidor
498-505
Publikation/Tidskrift/Serie
Journal of Antimicrobial Chemotherapy
Volym
57
Issue
3
Dokumenttyp
Artikel i tidskrift
Förlag
Oxford University Press
Ämne
- Pharmacology and Toxicology
Nyckelord
- drug resistance
- wild-type MIC distributions
- clinical microbiology
- antimicrobial susceptibility tests
- MIC breakpoints
Status
Published
Forskningsgrupp
- Clinical Microbiology, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1460-2091