Protein secretion from mouse skeletal muscle: coupling of increased exocytotic and endocytotic activities in denervated muscle
Författare
Summary, in English
The secretion of proteins labelled by incorporation of radioactive amino acids was studied in innervated and 10 to 13-day-denervated mouse skeletal muscle. The secretion of 3H-leucine-labelled proteins, expressed per mg muscle wet weight, increased after denervation, and the kinetics of the secretory process was also altered in denervated muscle. Separation of secreted 35S-methionine-labelled proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis followed by autoradiography revealed some denervation-induced alterations in the pattern of secreted proteins. The secretion from both innervated and denervated muscle was highly temperature sensitive and was reversibly inhibited by brefeldin A, a drug that blocks forward membrane transport from the endoplasmic reticulum/Golgi apparatus. This drug was also found to inhibit the uptake of fluorescein isothiocyanate-labelled dextran in denervated muscle but had no effect on the endocytotic activity of innervated muscle. This lends support to the hypothesis that the increased endocytotic activity in denervated muscle is coupled to a high secretory activity.
Avdelning/ar
Publiceringsår
1993
Språk
Engelska
Sidor
49-56
Publikation/Tidskrift/Serie
Cell and Tissue Research
Volym
274
Issue
1
Dokumenttyp
Artikel i tidskrift
Förlag
Springer
Ämne
- Cell Biology
Nyckelord
- Muscle
- skeletal
- Exocytosis
- Endocytosis
- Denervation
- Brefeldin A
- Mouse (NMRI)
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1432-0878