An immunochemical method for quantitative determination of latent antithrombin, the reactive center loop-inserted uncleaved form of antithrombin.
Författare
Summary, in English
Antithrombin (AT) is a serine protease inhibitor that has thrombin, factors IXa and Xa as target proteases. In addition to active native AT, two other forms have been identified in plasma: the reactive center loop inserted cleaved and latent, uncleaved forms. Both have been shown to be present in normal human blood. Latent AT forms a dimer with native AT in vitro, thus inactivating the native form. Here we describe a mouse monoclonal antibody, 8C8, that is specific for latent AT. The affinity of 8C8 was found to be 500-fold higher for latent than for native AT and 5000-fold higher for latent than for cleaved AT. A sandwich assay was developed to measure the concentration of latent AT in plasma, which was found to be similar to 4.8 mg L-1 in healthy individuals. The K-D of the interaction between native and latent AT was found to be 51 mu M, i.e. far above the plasma concentration of both native and latent AT, indicating a negligible complex formation in blood.
Avdelning/ar
Publiceringsår
2007
Språk
Engelska
Sidor
127-132
Publikation/Tidskrift/Serie
Journal of Thrombosis and Haemostasis
Volym
5
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Wiley-Blackwell
Ämne
- Cardiac and Cardiovascular Systems
Nyckelord
- latent antithrombin
- immunochemical sandwich assay
- antithrombin
- monoclonal antibody
- native-latent antithrombin dimer
Status
Published
Forskningsgrupp
- Clinical Chemistry, Malmö
- Celiac Disease and Diabetes Unit
ISBN/ISSN/Övrigt
- ISSN: 1538-7933