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DRhoGEF2 Regulates Cellular Tension and Cell Pulsations in the Amnioserosa during Drosophila Dorsal Closure

In English

Författare

  • Dulce Azevedo
  • Marco Antunes
  • Soren Prag
  • Xiaoyan Ma
  • Udo Häcker
  • G. Wayne Brodland
  • M. Shane Hutson
  • Jerome Solon
  • Antonio Jacinto

Summary, in English

Coordination of apical constriction in epithelial sheets is a fundamental process during embryogenesis. Here, we show that DRhoGEF2 is a key regulator of apical pulsation and constriction of amnioserosal cells during Drosophila dorsal closure. Amnioserosal cells mutant for DRhoGEF2 exhibit a consistent decrease in amnioserosa pulsations whereas overexpression of DRhoGEF2 in this tissue leads to an increase in the contraction time of pulsations. We probed the physical properties of the amnioserosa to show that the average tension in DRhoGEF2 mutant cells is lower than wild-type and that overexpression of DRhoGEF2 results in a tissue that is more solid-like than wild-type. We also observe that in the DRhoGEF2 overexpressing cells there is a dramatic increase of apical actomyosin coalescence that can contribute to the generation of more contractile forces, leading to amnioserosal cells with smaller apical surface than wild-type. Conversely, in DRhoGEF2 mutants, the apical actomyosin coalescence is impaired. These results identify DRhoGEF2 as an upstream regulator of the actomyosin contractile machinery that drives amnioserosa cells pulsations and apical constriction.

Publiceringsår

2011

Språk

Engelska

Publikation/Tidskrift/Serie

PLoS ONE

Volym

6

Issue

9

Dokumenttyp

Artikel i tidskrift

Förlag

Public Library of Science (PLoS)

Ämne

  • Developmental Biology

Status

Published

Forskningsgrupp

  • Invertebrate Developmental Biology, Udo Haecker's group

ISBN/ISSN/Övrigt

  • ISSN: 1932-6203