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A reduced population of CD103+CD11b+ dendritic cells has a limited impact on oral Salmonella infection

In English

Författare

  • María Fernández-Santoscoy
  • Ulf Alexander Wenzel
  • Emma Persson
  • Ulf Yrlid
  • William Agace
  • Mary Jo Wick

Summary, in English

CD103+CD11b+ dendritic cells (DC) are the major migratory DC subset in the small intestine lamina propria (siLP) and their survival is dependent on the transcription factor interferon regulatory factor 4 (IRF4). Mice with a DC-specific deletion of irf4 (CD11c-cre.Irf4 mice) have reduced mucosal CD103+CD11b+ DC and altered T cell differentiation to protein antigen. The influence of CD103+CD11b+ DC on oral infection with the gastrointestinal pathogen Salmonella, however, is poorly understood and is investigated here. We show that, despite being infected with Salmonella, CD11c-cre.Irf4 mice (called Cre+ mice) conserve the reduction in CD103+CD11b+ DC observed in naive Cre+ mice, particularly in the mesenteric lymph nodes (MLN) but also in the siLP at day 3 post infection. Moreover, Salmonella-infected Cre+ mice have a similar bacterial burden in intestinal tissues (siLP, MLN and Peyer's patches) as well as the spleen compared to infected Cre- controls. The T cell compartment, including the frequency of IFN-γ and IL-17-producing T cells, is not altered in intestinal tissues of Salmonella-infected Cre+ mice relative to infected Cre- controls. In addition, no difference between infected Cre+ and Cre- mice was observed in either the concentration of IL-6 or IL-17 in whole tissue lysates of siLP, MLN or Peyer's patches or in the serum concentration of Salmonella-specific IgG and IgM. Overall the data suggest that the reduction of CD103+CD11b+ DC in Cre+ mice has little if any impact on Salmonella burden in infected tissues or eliciting effector functions important in host survival at later stages of the infection.

Avdelning/ar

Publiceringsår

2016-08-01

Språk

Engelska

Sidor

72-80

Publikation/Tidskrift/Serie

Immunology Letters

Volym

176

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Immunology in the medical area

Nyckelord

  • CD103
  • Dendritic cells
  • Infection
  • IRF4
  • Mesenteric lymph node
  • Salmonella

Status

Published

Forskningsgrupp

  • Immunology
  • Mucosal Immunology

ISBN/ISSN/Övrigt

  • ISSN: 0165-2478