A divalent antibody format is required for neutralization of human cytomegalovirus via antigenic domain 2 on glycoprotein B

Summary, in English

Glycoprotein B (gB) of human cytomegalovirus (HCMV) is the dominating protein in the envelope of this virus and gives rise to virus-neutralizing antibodies in most infected individuals. We have previously isolated a neutralizing human antibody specific for antigenic domain 2 (AD-2) on gB, a poorly immunogenic epitope, which nevertheless is capable of eliciting potent neutralizing antibodies. In order to define parameters important for the neutralization of HCMV via gB, we have investigated the virus-neutralizing capacity and the kinetics of the interaction with AD-2 of the monomeric and dimeric forms of a single chain variable fragment (scFv) corresponding to this antibody. We demonstrate here that neutralization of HCMV via AD-2 on gB can be mediated by dimeric scFv, while monomeric fragments cannot mediate neutralization of the virus, despite a slow dissociation from the intact glycoprotein. This finding is discussed in the context of possible mechanisms for antibody-mediated virus neutralization.

• Författare: Johan Lantto; Jean M Fletcher; Mats Ohlin
• Avdelning/ar: Institutionen för immunteknologi
• Publiceringsår: 2002
• Språk: Engelska
• Sidor: 2001-2005
• Publikation/Tidskrift/Serie: Journal of General Virology
• Volym: 83
• Issue: Pt 8
• Fulltext: - 286 kB; Download statistics
• Länkar: http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12124464&dopt=Abstract
• Dokumenttyp: Artikel i tidskrift
• Förlag: Microbiology Society
• Ämne: Immunology in the medical area
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1465-2099