A new expression vector for production of enzymes in the yeast Saccharomyces (lachancea) kluyveri.
Publikation/Tidskrift/Serie: Nucleosides, Nucleotides & Nucleic Acids
Dokumenttyp: Artikel i tidskrift
Förlag: Taylor & Francis
We overexpressed and purified enzymes involved in the pyrimidine catabolic pathway in the yeast Saccharomyces (Lachancea) kluyveri. A new vector was therefore designed, providing the first specific expression system in Saccharomyces kluyveri. The URC1 gene was overexpressed and a soluble protein obtained and successfully purified using the C-terminally added His-tag. Our system will be used for further studies of the structure and function of the enzymes belonging to the URC pyrimidine degradation pathway.
- Biological Sciences
- Expression vector
- Saccharomyces (Lachancea) kluyveri
- URC pathway
- catabolism of pyrimidines
- ISSN: 1525-7770