A new expression vector for production of enzymes in the yeast Saccharomyces (lachancea) kluyveri.
Författare
Summary, in English
We overexpressed and purified enzymes involved in the pyrimidine catabolic pathway in the yeast Saccharomyces (Lachancea) kluyveri. A new vector was therefore designed, providing the first specific expression system in Saccharomyces kluyveri. The URC1 gene was overexpressed and a soluble protein obtained and successfully purified using the C-terminally added His-tag. Our system will be used for further studies of the structure and function of the enzymes belonging to the URC pyrimidine degradation pathway.
Avdelning/ar
Publiceringsår
2011
Språk
Engelska
Sidor
1227-1232
Publikation/Tidskrift/Serie
Nucleosides, Nucleotides & Nucleic Acids
Volym
30
Issue
12
Dokumenttyp
Artikel i tidskrift
Förlag
Taylor & Francis
Ämne
- Biological Sciences
Nyckelord
- Expression vector
- Saccharomyces (Lachancea) kluyveri
- URC pathway
- catabolism of pyrimidines
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1525-7770